prevalence of blaoxa10 type beta-lactamase gene in carbapenemase producing pseudomonas aeruginosa strains isolated from patients in isfahan

background pseudomonas aeruginosa is a leading cause of infections especially in patients with weakened immune systems. this pathogen is intrinsically resistant to many antibacterial agents. objectives the aim of this study was to survey the antibiotic resistance pattern and frequency of bla oxa10 gene in carbapenemase producing p. aeruginosa isolated from patients in isfahan hospitals. materials and methods total of 100 clinical isolates of p. aeruginosa from different samples were collected. antibiotic susceptibility for antimicrobial agents was carried out according to the clinical and laboratory standards institute (clsi) guidelines, and the frequency of the gene encoding bla oxa10 was studied by polymerase chain reaction (pcr). results the resistance rate of the isolated strains to certain antibiotics wasas following: ciprofloxacin (56%), gentamicin (59%), tobramycin (61%), amikacin (65%), imipenem (55%), cefepime (55%), ceftazidime (57%), ceftriaxone (60%), cefotaxime (62%), oxacillin (100%), and piperacillin (48%), respectively. p. aeruginosa demonstrated the highest resistance rate to oxacillin (100 %), and 55% of isolates were resistant to imipenem and cefepime, whereas 63% were multidrug-resistant (resistant to three or more classes of antibiotics). a mdr phenotype occurred frequently in p. aeruginosa. pcr was performed for all the resistant strains, where the frequency of blaoxa10 gene was 40 (64%). conclusions our results showed that the prevalence of mdr and extended-spectrum β lactamases producing p. aeruginosa in isfahan was very high and proper infection control practices are essential to prevent the spreading of esbl-producing p. aeruginosa in hospitals.